Associations of Intensity, Volume, and Fragmentation of Physical Activity With Mortality Risk

Table of Contents

Overall Summary

Overview

This study investigated the associations of physical activity (PA) intensity, volume, duration, and fragmentation with all-cause and cardiovascular disease (CVD) mortality risk in US adults using data from the National Health and Nutrition Examination Survey (NHANES). The study also aimed to establish reference values for PA volume and intensity within the US adult population by creating centile curves.

Key Findings

Strengths

Areas for Improvement

Significant Elements

Figure 1

Description: This figure illustrates the dose-response relationship between PA intensity/volume (IG and AvAcc) and all-cause mortality risk, showing a plateau effect beyond certain levels.

Relevance: It visually demonstrates the key finding that both higher intensity and greater volume of PA are associated with a lower risk of all-cause mortality, up to a certain point.

Figure 3

Description: This figure presents age- and sex-specific centile curves for AvAcc and IG in the US adult population, highlighting the decline in PA with age.

Relevance: It provides valuable reference values for assessing PA levels and understanding the age-related trends in PA intensity and volume.

Conclusion

This study highlights the importance of PA intensity for reducing mortality risk in US adults. Higher PA intensity, rather than just volume, is associated with lower all-cause and CVD mortality. Accumulating intense PA in continuous bouts may be more beneficial than fragmented PA. The generated centile curves offer valuable benchmarks for assessing PA levels and informing personalized recommendations to promote health and longevity.

Section Analysis

Aims

Overview

This section concisely outlines the two main goals of the research study. The first aim is to explore the relationship between various aspects of physical activity (volume, intensity, duration, and fragmentation) and mortality risk, specifically focusing on all-cause and cardiovascular disease mortality. The second aim is to establish reference values for physical activity volume and intensity within the US adult population by creating centile curves.

Key Aspects

Strengths

Suggestions for Improvement

Methods and results

Overview

This section details the methodology employed in the study, including the data source, population, outcome measures, accelerometer data processing, physical activity metrics, and statistical analysis techniques. It then presents the key findings regarding the association of physical activity intensity, volume, duration, and fragmentation with mortality risk, as well as the development of reference values and centile curves for physical activity volume and intensity.

Key Aspects

Strengths

Suggestions for Improvement

Graphical Abstract

Overview

This section visually summarizes the key findings of the study regarding the association of physical activity (PA) with mortality risk. It uses infographics to illustrate the relationships between PA volume, intensity, duration, and fragmentation with all-cause and cardiovascular disease mortality. The graphical abstract highlights the importance of PA intensity and its distribution for longevity, suggesting that accumulating intense PA in continuous bouts may be more beneficial than doing it sporadically.

Key Aspects

Strengths

Suggestions for Improvement

Non-Text Elements

Figure Graphical Abstract - Associations of PA volume, intensity, and duration with mortality

This infographic, titled 'Associations of PA volume, intensity, and duration with mortality,' visually depicts the relationship between physical activity (PA) characteristics and mortality risk. It is divided into three sections: Intensity Distribution, Intensity Gradient, and Average Acceleration. The Intensity Distribution section uses a scale to qualitatively compare the predictive power of intensity versus volume, suggesting intensity as a stronger predictor. The Intensity Gradient section presents a graph showing the hazard ratio against the intensity gradient, accompanied by two figures illustrating different intensity distributions. The Average Acceleration section displays a graph of the hazard ratio against average acceleration, along with two figures representing different PA volumes.

First Mention

Text: ""

Context:

Relevance: This infographic serves as a visual summary of the study's core findings, highlighting the importance of PA intensity and volume in relation to mortality risk. It provides a readily accessible overview of the complex relationships explored in the main text.

Critique
Visual Aspects
  • The infographic effectively uses a combination of scales, graphs, and figures to convey information in a concise and visually appealing manner.
  • The color scheme is simple and intuitive, with clear distinctions between different levels of intensity and volume.
  • The layout is well-organized, guiding the viewer through the different sections of the infographic.
Analytical Aspects
  • The infographic clearly demonstrates the inverse relationship between PA intensity/volume and mortality risk, supporting the study's main findings.
  • The use of hazard ratios provides a quantitative measure of the risk reduction associated with different PA levels.
  • The inclusion of figures illustrating different intensity distributions and volumes helps to contextualize the findings and make them more relatable to the reader.
Numeric Data
  • Hazard Ratio Reduction (Intensity Gradient - 25th to 50th percentile): -37.1 %
  • Hazard Ratio Reduction (Intensity Gradient - 50th to 75th percentile): -18.8 %
  • Hazard Ratio Reduction (Average Acceleration - 25th to 50th percentile): -14.4 %
  • Hazard Ratio Reduction (Average Acceleration - 50th to 75th percentile): -13.7 %
  • Intensity Gradient Plateau: -2.7
  • Average Acceleration Plateau: 35.0 mg
Figure Graphical Abstract - Associations of PA fragmentation with mortality

This infographic, titled 'Associations of PA fragmentation with mortality,' focuses on the impact of how physical activity is distributed throughout the day on mortality risk. It presents two scenarios: 'More in bouts' (e.g., 1 x 5 min moderate run) and 'More sporadically' (e.g., 10 x 30 s moderate run). A scale visually indicates that accumulating the most intense PA in continuous bouts is associated with a lower mortality risk compared to accumulating the same amount of PA in shorter, more fragmented bursts.

First Mention

Text: ""

Context:

Relevance: This infographic complements the previous one by specifically addressing the role of PA fragmentation in mortality risk. It visually reinforces the study's finding that continuous bouts of intense PA may be more beneficial than fragmented activity, even when the total volume and intensity are similar.

Critique
Visual Aspects
  • The infographic effectively uses simple figures and a scale to illustrate the concept of PA fragmentation and its impact on mortality risk.
  • The contrasting scenarios ('More in bouts' vs. 'More sporadically') are clearly presented and easy to understand.
  • The color scheme is consistent with the previous infographic, maintaining visual coherence.
Analytical Aspects
  • The infographic highlights the importance of considering not only the volume and intensity of PA but also how it is distributed throughout the day.
  • The visual representation of the scale effectively conveys the message that continuous bouts of intense PA are associated with a lower mortality risk.
  • The infographic could be strengthened by providing a more quantitative measure of the risk reduction associated with different fragmentation patterns.
Numeric Data

Keywords

Overview

This section lists keywords relevant to the research paper, aiming to improve its discoverability and categorization within academic databases and search engines. These keywords reflect the core themes and methodologies of the study, including the use of accelerometers for physical activity measurement, the generation of normative data, and the investigation of longevity.

Key Aspects

Strengths

Suggestions for Improvement

Introduction

Overview

This section introduces the importance of physical activity (PA) for longevity and the role of accelerometers in understanding this relationship. It highlights the emerging focus on PA intensity in addition to volume, introduces the metrics of average acceleration (AvAcc) and intensity gradient (IG), and identifies gaps in the current understanding of PA's impact on mortality, particularly regarding the intensity spectrum and fragmentation. The section concludes by stating the study's two main aims: investigating the association of various PA aspects with mortality risk and producing representative centile curves for AvAcc and IG in US adults.

Key Aspects

Strengths

Suggestions for Improvement

Methods

Overview

This section outlines the methodology employed to investigate the associations between physical activity (PA) and mortality, as well as to generate reference values for PA volume and intensity. It describes the data source, participant selection criteria, outcome measures, accelerometer data processing, PA metrics used, and statistical analysis techniques.

Key Aspects

Strengths

Suggestions for Improvement

Results

Overview

This section presents the findings of the study, focusing on the associations between physical activity (PA) metrics and mortality risk, as well as the development of reference values for PA volume and intensity in the US adult population. The results indicate that PA intensity, particularly the intensity gradient (IG), is a stronger predictor of both all-cause and cardiovascular disease (CVD) mortality than PA volume (average acceleration, AvAcc). The study also found that accumulating intense PA in continuous bouts is associated with lower mortality risk compared to fragmented PA. Age- and sex-specific centile curves for AvAcc and IG are presented, highlighting the decline in PA intensity and volume with age.

Key Aspects

Strengths

Suggestions for Improvement

Non-Text Elements

Table Table 1

Table 1, titled 'Population characteristics stratified by sex derived from specific weighting of cohort data,' presents demographic and health characteristics of the study participants, stratified by sex. It includes information on age, body mass index, ethnicity, education level, household income, prevalent health conditions (congestive heart failure, coronary heart disease, heart attack, angina pectoris, stroke, cancer, diabetes), and physical activity measures (average acceleration, intensity gradient, total physical activity, inactivity, and fragmentation ratios). The table provides median values with 25th and 75th percentiles or relative frequencies for each characteristic. For example, the median age for women was 49 years (36, 62), and the median average acceleration for men was 33.0 mg (25.5, 42.4).

First Mention

Text: "Table 1 shows sex-stratified population characteristics and Supplementary material online, Figure S1 the flow of participants."

Context: This sentence, appearing early in the 'Results' section, introduces Table 1 and directs the reader to supplementary materials for further details on participant characteristics.

Relevance: Table 1 provides a comprehensive overview of the study population's baseline characteristics, allowing readers to understand the demographic and health profile of the participants. This information is crucial for interpreting the study's findings and assessing their generalizability.

Critique
Visual Aspects
  • The table is well-organized and easy to read, with clear headings and subheadings that guide the reader through the data.
  • The use of both medians and percentiles provides a good understanding of the distribution of the data for each characteristic.
  • The table could benefit from using color or shading to highlight key findings or differences between sex groups.
Analytical Aspects
  • The use of specific weighting to match population counts from the Census Bureau enhances the representativeness of the sample.
  • The inclusion of a wide range of demographic and health characteristics allows for a thorough assessment of potential confounding factors.
  • The table could be strengthened by providing statistical comparisons between sex groups, such as p-values or effect sizes, to highlight any significant differences.
Numeric Data
  • Median Age (Women): 49 years
  • Median Average Acceleration (Men): 33.0 mg
  • Prevalence of Diabetes (Overall): 10.4 %
  • Median Total Physical Activity (Women): 370 min.d-1
  • Median M60RATIO (Men): 0.34
Figure Figure 1

Figure 1, titled 'Dose–response plot of intensity gradient (A) or average acceleration (B) with all-cause mortality,' presents two graphs illustrating the relationship between physical activity intensity/volume and the risk of all-cause mortality. Graph A shows a curvilinear inverse relationship between the intensity gradient (IG) and hazard ratio, plateauing at an IG range of -2.7 to -2.5. Graph B depicts a similar inverse relationship between average acceleration (AvAcc) and hazard ratio, stabilizing at approximately 35-45 mg. Both graphs include histograms showing the distribution of the underlying data and vertical lines marking the 25th, 50th, and 75th percentiles. The shaded red areas represent 95% confidence intervals.

First Mention

Text: "An inverse curvilinear relationship with all-cause mortality was ob- served for both IG, plateauing at −2.7 to −2.5, and AvAcc, stabilizing at ∼35–45 mg, beyond which no further risk alterations were noted (Figure 1A and B)."

Context: This paragraph, located in the 'Results' section under the subheading 'Association of intensity, volume, and duration of PA with mortality risk,' describes the dose-response relationship between physical activity intensity/volume and all-cause mortality, referencing Figure 1.

Relevance: Figure 1 visually demonstrates the key finding that both higher intensity (IG) and greater volume (AvAcc) of physical activity are associated with a lower risk of all-cause mortality, up to a certain point. The plateauing effect suggests that there may be an optimal range of intensity and volume for maximizing health benefits.

Critique
Visual Aspects
  • The use of separate graphs for IG and AvAcc allows for a clear comparison of the dose-response relationships for each metric.
  • The inclusion of histograms and percentile markers provides a good understanding of the distribution of the data.
  • The figure could be improved by adding units to the x-axis of Graph A (Intensity Gradient) for clarity.
Analytical Aspects
  • The use of natural splines to model the dose-response relationships allows for the capture of non-linear patterns.
  • The presentation of 95% confidence intervals provides a measure of uncertainty around the estimated hazard ratios.
  • The figure could be strengthened by including statistical tests to assess the significance of the observed relationships.
Numeric Data
  • Intensity Gradient Plateau: -2.7
  • Average Acceleration Plateau: 35 mg
  • Hazard Ratio Reduction (IG - 25th to 50th percentile): -37.1 %
  • Hazard Ratio Reduction (AvAcc - 25th to 50th percentile): -14.4 %
  • Hazard Ratio Reduction (IG - 50th to 75th percentile): -18.8 %
Figure Figure 2

Figure 2, titled 'Physical activity intensity profile stratified by hazard ratio,' presents two radar charts illustrating the intensity profile of physical activity across different hazard ratio groups. The left chart displays the data on the original scale, while the right chart shows z-transformed data. Both charts are stratified by hazard ratio (HR) and depict the acceleration levels associated with the most active minutes within a day, ranging from M720 (most active 720 minutes) to M1 (most active 1 minute). The shaded areas represent 95% confidence intervals. The left chart also includes dashed black circles indicating acceleration levels corresponding to slow walking (100 mg), brisk walking (200 mg), and fast walking (400 mg).

First Mention

Text: "MX plots (Figure 2) show the intensity difference in PA profiles across three hazard ratio groups."

Context: This sentence, appearing in the 'Results' section under the subheading 'Association of intensity, volume, and duration of PA with mortality risk,' introduces Figure 2 and explains its purpose.

Relevance: Figure 2 provides a detailed visualization of the physical activity patterns associated with different levels of mortality risk. It highlights the finding that individuals with lower hazard ratios tend to engage in more intense physical activity, particularly for shorter durations, compared to those with higher hazard ratios.

Critique
Visual Aspects
  • The use of radar charts effectively illustrates the intensity profile of physical activity across different time scales.
  • The stratification by hazard ratio allows for a clear comparison of activity patterns between risk groups.
  • The figure could be improved by providing a clearer explanation of the MX metric and its interpretation in the caption or figure legend.
Analytical Aspects
  • The inclusion of 95% confidence intervals provides a measure of uncertainty around the estimated acceleration levels.
  • The use of both original and z-transformed data allows for a comprehensive visualization of the intensity profiles.
  • The figure could be strengthened by including statistical tests to assess the significance of the differences between hazard ratio groups.
Numeric Data
  • Slow Walking Acceleration: 100 mg
  • Brisk Walking Acceleration: 200 mg
  • Fast Walking Acceleration: 400 mg

Discussion

Overview

This section discusses the study's key findings regarding the association of physical activity (PA) intensity and volume with mortality risk in US adults. It highlights that PA intensity, as reflected by the intensity gradient (IG), is a stronger predictor of both all-cause and cardiovascular disease (CVD) mortality than PA volume (average acceleration, AvAcc). The discussion also emphasizes the importance of accumulating intense PA in continuous bouts rather than sporadically for reducing mortality risk. The section further discusses the generated centile curves for AvAcc and IG, which offer benchmarks for assessing PA levels in the US adult population and provide a basis for personalized recommendations. The limitations of the study are also acknowledged, including the cross-sectional design and potential biases related to accelerometer calibration errors.

Key Aspects

Strengths

Suggestions for Improvement

Non-Text Elements

Table Table 2

Table 2, titled 'Association of the fragmentation of physical activity (z-transformed) with all-cause and cardiovascular disease mortality,' examines the relationship between physical activity fragmentation and mortality risk. It presents two models: Model 1 focuses on the intensity gradient, while Model 2 focuses on average acceleration. For each model, the table shows the likelihood ratio test (LRT) p-value, hazard ratio (HR), and 95% confidence interval (CI) for three metrics of fragmentation: M60RATIO, M15RATIO, and M5RATIO. These metrics represent the ratio of the intensity of the most active continuous 60, 15, and 5 minutes, respectively, to the intensity of the same duration accumulated in fragmented segments. For instance, in Model 1 for all-cause mortality, the M15RATIO has an LRT p-value of 0.007, a hazard ratio of 0.82, and a 95% CI of 0.68–0.88. This suggests that greater fragmentation of the most active 15 minutes is associated with a higher risk of all-cause mortality.

First Mention

Text: "Yet, with AvAcc, only M15RATIO improved the prediction. Regarding CVD mortality, long- er MXRATIO periods were more relevant for the prediction when com- bined with IG. Combined with AvAcc, MXRATIO did not add to the prediction (Table 2)."

Context: This paragraph, appearing in the 'Results' section under the subheading 'Association of PA fragmentation with mortality risk,' discusses the findings related to physical activity fragmentation and its impact on predicting mortality risk. It specifically mentions that the M15RATIO metric improved prediction with average acceleration (AvAcc) and that longer MXRATIO periods were more relevant when combined with the intensity gradient (IG). The sentence directly preceding the mention of Table 2 states that MXRATIO did not improve prediction when combined with AvAcc for CVD mortality.

Relevance: Table 2 supports the study's finding that physical activity fragmentation is associated with mortality risk. It provides statistical evidence that, for a given intensity and volume of physical activity, accumulating the most intense activity in continuous bouts is associated with a lower risk of mortality compared to spreading it out in fragmented segments. This finding challenges the notion that 'every minute counts' equally, suggesting that the pattern of activity accumulation may also be important.

Critique
Visual Aspects
  • The table is well-structured and easy to read, with clear headings and subheadings separating the different models and outcome variables.
  • The use of abbreviations (LRT, HR, CI) is consistent with standard statistical reporting and is explained in the table footnote.
  • The table could be enhanced by visually highlighting significant p-values or hazard ratios that fall outside the null range.
Analytical Aspects
  • The use of the likelihood ratio test (LRT) provides a statistically rigorous method for assessing whether the addition of MXRATIO improves the model fit.
  • The inclusion of both intensity gradient and average acceleration models allows for a comparison of the association between fragmentation and mortality across different measures of physical activity.
  • The table could be strengthened by providing a more detailed interpretation of the findings, discussing the magnitude of the hazard ratios and the clinical significance of the observed associations.
Numeric Data
  • LRT p-value (Model 1, All-Cause Mortality, M15RATIO): 0.007
  • Hazard Ratio (Model 1, All-Cause Mortality, M15RATIO): 0.82
  • Lower Bound of 95% CI (Model 1, All-Cause Mortality, M15RATIO): 0.68
  • Upper Bound of 95% CI (Model 1, All-Cause Mortality, M15RATIO): 0.88
  • LRT p-value (Model 2, CVD Mortality, M60RATIO): 0.46
Figure Figure 3

Figure 3, titled 'Reference values for volume and intensity of physical activity,' presents age- and sex-specific centile curves for average acceleration (AvAcc) and intensity gradient (IG) in the adult US population. The figure is divided into four panels: panels A and B display AvAcc curves for males and females, respectively, while panels C and D show IG curves for males and females, respectively. Each panel presents centile curves for the 5th, 10th, 15th, 25th, 50th, 75th, 85th, 90th, and 95th percentiles. The x-axis represents age (in years), ranging from approximately 20 to 90, and the y-axis represents either AvAcc (in mg) or IG. The curves are color-coded to reflect the dose-response relationship with all-cause mortality, with green indicating a reduced hazard ratio and red indicating an increased hazard ratio. The caption specifies that a reduced hazard ratio is defined as IG: -2.7 to -2.5 and AvAcc: ~35-45 mg. For example, in panel A (males - AvAcc), the 50th percentile curve starts at approximately 40 mg at age 20 and gradually declines to approximately 25 mg at age 90. The color of the curve transitions from green to red around age 50, suggesting that AvAcc levels below this threshold are associated with an increased risk of all-cause mortality in older men.

First Mention

Text: "Age- and sex-specific centile curves were generated for AvAcc and IG (Figure 3)."

Context: This sentence, appearing in the 'Results' section under the subheading 'Reference values and centile curves,' introduces Figure 3 and states that it presents age- and sex-specific centile curves for average acceleration (AvAcc) and intensity gradient (IG).

Relevance: Figure 3 provides valuable reference values for PA volume and intensity in the US adult population, allowing individuals and clinicians to compare their PA levels to those associated with reduced mortality risk. The figure highlights the decline in both PA intensity and volume with age, emphasizing the importance of maintaining adequate PA levels throughout adulthood.

Critique
Visual Aspects
  • The figure is well-organized and easy to understand, with clear labels for each panel, axis, and centile curve.
  • The color scheme effectively conveys the dose-response relationship with mortality, although a version for colorblind individuals is mentioned as being available in supplementary material.
  • The figure could be enhanced by adding shaded areas around the curves to represent confidence intervals, providing a visual indication of the uncertainty around the estimated percentiles.
Analytical Aspects
  • The use of centile curves provides a comprehensive representation of the distribution of PA volume and intensity across the population.
  • The color-coding based on the dose-response relationship with mortality adds valuable information, highlighting PA levels associated with different risk levels.
  • The figure could be strengthened by providing more specific information about the statistical methods used to generate the curves and the criteria for defining the color thresholds.
Numeric Data
  • Reduced Hazard Ratio Threshold (IG): -2.7
  • Reduced Hazard Ratio Threshold (AvAcc): 35 mg
  • 50th Percentile AvAcc (Males, Age 20): 40 mg
  • 50th Percentile AvAcc (Males, Age 90): 25 mg
  • Age at Color Transition (Males, AvAcc): 50 years

Limitations

Overview

This section acknowledges the limitations of the study, primarily focusing on the inherent constraints of the cross-sectional design and potential biases stemming from accelerometer calibration errors in the NHANES dataset. While acknowledging these limitations, the authors argue that the study's findings remain robust and comparable to previous research.

Key Aspects

Strengths

Suggestions for Improvement

Conclusions

Overview

This section summarizes the main findings of the study, emphasizing the importance of physical activity (PA) intensity for reducing mortality risk. It concludes that higher PA intensity, rather than just volume, is associated with lower all-cause and cardiovascular disease (CVD) mortality. The section also highlights the potential benefit of accumulating intense PA in continuous bouts, suggesting it may be more effective than spreading the same amount of activity throughout the day. The authors propose the generated centile curves as benchmarks for evaluating PA levels and guiding personalized recommendations.

Key Aspects

Strengths

Suggestions for Improvement

Lay summary

Overview

This section provides a simplified explanation of the study's main findings for a general audience. It emphasizes that the intensity of physical activity, rather than the total volume, is more strongly associated with a reduced risk of death, particularly from cardiovascular disease. The section also highlights the potential benefit of engaging in continuous bouts of intense physical activity for health optimization.

Key Aspects

Strengths

Suggestions for Improvement

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